![]() Therefore, achieving a greater understanding of the genetics of salt tolerance in plants and its exploitation in improving salt tolerance of plants, especially crops, is one of the most important goals in China. As the largest province in northwest China, Xinjiang occupies one-sixth of the land of China however, more than one-third of the land in Xinjiang suffers from primary of secondary salinization ( Zhao et al., 2013). About 100 million hectares of land in China is exposed to primary salinity or secondary salinity, accounting for approximately 10% of the world’s saline and alkaline land resources. Soil salinity is one of the major abiotic factors constraining plant growth and crop production. Furthermore, soil salinization has increased in recent years as a result of anthropogenic actions such as irrigation.Īs sessile organisms, plants are subjected to various environmental stresses, such as drought, salinity, and temperature extremes, during their life cycle. Salinity is a serious problem worldwide, with more than 800 million hectares of land across the world estimated to be affected by high salinity ( Munns, 2005). pumila will facilitate the understanding of complex adaptive mechanisms for ephemerals. The rapid discovery of known or unknown genes related to salinity stress response in A. The large-scale EST library obtained in this study provides first-hand information on gene sequences expressed in young leaves of A. Lastly, changes in expression of the 12 most abundant unigenes, 12 transcription factor genes, and 19 stress-related genes in the first 24 h of exposure to high-salinity stress conditions were monitored by qRT-PCR. In addition, 251 transcription factors were identified from the library, classified into 42 families. Many previously reported salt tolerance genes were also manifested in this library, for example VP1, H +-ATPase, NHX1, SOS2, SOS3, NAC, MYB, ERF, LEA, P5CS1. At least seven main classes of genes were related to salt-tolerance among the 8,835 unigenes. Functional classification demonstrated an abundance of unigenes involved in binding, catalytic, structural or transporter activities, and in pathways of energy, carbohydrate, amino acid, or lipid metabolism. The blastx results revealed 8,011 unigenes with significant similarity to known genes, while only 425 unigenes remained uncharacterized. Clustering and assembly of these ESTs resulted in the identification of 8,835 unique sequences, consisting of 2,469 contigs and 6,366 singletons. A total of 16,014 high-quality expressed sequence tags (ESTs) were generated, which have been deposited in the dbEST GenBank under accession numbers JZ932319 to JZ948332. To improve our understanding of the molecular and genetic mechanisms of salt stress adaptation, more than 19,900 clones randomly selected from a cDNA library constructed previously from leaf tissue exposed to high-salinity shock were sequenced. pumila is an under-explored resource for understanding the genetic mechanisms underlying abiotic stress adaptation. thaliana, thus providing a candidate plant system for gene mining for environmental adaption and salt tolerance. Special Plant Genomics Laboratory, College of Life Sciences, Shihezi University, Shihezi, ChinaĪrabidopsis pumila is an ephemeral plant, and a close relative of the model plant Arabidopsis thaliana, but it possesses higher photosynthetic efficiency, higher propagation rate, and higher salinity tolerance compared to those A.Xianzhong Huang *†, Lifei Yang †, Yuhuan Jin, Jun Lin and Fang Liu
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |